Plant Protect. Sci., 2002, 38(11):714-716 | DOI: 10.17221/10597-PPS

Molecular and genetic studies of the basis of virulence/avirulence in Meloidogyne chitwoodi

V.C. Blok, J. Wishart, D. Mugniéry, M. Bossis, P. Castagnone-Sereno, C. Neveu, G. Smant, C. Zijlstra, F.C. Zoon, M.S. Phillips
1 Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA United Kingdom
2 Zoology Research Laboratory INRA de Rennes,
5 160 Le Rheu, France
3 INRA Units of Plant Microbe Interactions and Plant Health, 06606 Antibes Cedex, France
4 Department of Plant Sciences, Wageningen University and Research Centre, 6709 PD Wageningen, The Nether- lands
5 Plant Research International, 6700 AA Wageningen, The Netherlands E-mail: mphill@scri.sari.ac.uk$3

Within the EU project DREAM there is a programme of work investigating the molecular basis of virulence and avirulence in Meloidogyne chitwoodi and which involves partners from the Netherlands, France and Scotland. In Wageningen AFLPs are being used to examine genetic diversity between populations of M. chitwoodi. Partners in Wageningen and INRA are using M. javanica and M. incognita as model systems to isolate genes producing secreted proteins that could be implicated in the host pathogen interaction. They will search for homologous sequences in M. chitwoodi. At Rennes two-dimensional electrophoresis (2DE) studies are being conducted. The PCR based suppressive subtractive hybridisation (SSH), has been used at PRI and SCRI to compare avirulent and virulent nematodes and infected plant material (resistant and susceptible) at different times after infection.

Keywords: Meloidogyne chitwoodi; potato; AFLP; cDNA; suppressive subtractive hybridisation (SSH); two-dimensional electrophoresis (2DE)

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Blok VC, Wishart J, Mugniéry D, Bossis M, Castagnone-Sereno P, Neveu C, et al.. Molecular and genetic studies of the basis of virulence/avirulence in Meloidogyne chitwoodi. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):714-716. doi: 10.17221/10597-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. QIN L., PRINS P., JONES J.T., POPEIJUS H., SMANT G., BAKKER J., HELDER J. (2001): GenEST, a powerful link between cDNA sequence data and gene expression profiles generated by cDNA-AFLP. Nucleic Acids Res., 29: 1616-1622. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content