Plant Protect. Sci., 2002, 38(11):638-641 | DOI: 10.17221/10578-PPS

Assessment of orange fruit colonization by biocontrol yeasts

V. Coco, V. Grimaldi, A. Campisano, L. Strano, A. Catara
1 DISTEF, Sez. Patologia Vegetale, Università
di Catania, 95123 Catania, Italy
2 Consorzio Catania Ricerche, 95131 Catania, Italy E-mail: cataraan@mbox.fagr.unict.it

A scanning electron microscope study of citrus fruits was performed to assess the colonization process of antagonistic yeasts to green mould of Tarocco oranges under artificial inoculation. Yeast suspensions of Debaryomyces hansenii DBVPG 4025, Pichia guilliermondii NRRL Y 18134, P. anomala J121 and Saccharomyces cerevisiae P1.6 with or without addition of 1% CaCl2 + 0.1% Tween 80 were evaluated. Penicillium digitatum was sprayed one hour later. Observation of fruit surface waxes revealed marked morphological diversity. Yeast cells and conidia were mostly localized on epicuticular waxes, particularly in naturally occurring small pits. The size of the wound affected the number of yeasts and conidia penetrating inside. Treatment with CaCl2 did not affect the waxes and/or the behaviour of the yeasts and pathogen.

Keywords: Penicillium digitatum; orange; Scanning Electron Microscopy; yeast; colonization

Published: December 31, 2002  Show citation

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Coco V, Grimaldi V, Campisano A, Strano L, Catara A. Assessment of orange fruit colonization by biocontrol yeasts. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):638-641. doi: 10.17221/10578-PPS.
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