Plant Protect. Sci., 2002, 38(11):615-616 | DOI: 10.17221/10570-PPS

Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging

A. Chini, S. Murray, J. Grant, C. Thomson, G. Loake
Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom

In order to identify components of the defence signalling network that may contribute to the establishment of disease resistance, we generated a novel PR-1::Luciferase transgenic line which was deployed in an imaging based screen to uncover novel defence-related mutants. Approximately, 5000 ethylmethane sulfonate (EMS) lines and 30 000 activation tagged lines were generated and screened for enhanced LUC activity via ultra low light imaging.

Keywords: signal transduction; luciferase imaging; systemic acquired resistance; Arabidopsis mutants

Published: December 31, 2002  Show citation

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Chini A, Murray S, Grant J, Thomson C, Loake G. Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):615-616. doi: 10.17221/10570-PPS.
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