Plant Protect. Sci., 2002, 38(11):528-530 | DOI: 10.17221/10545-PPS

Monitoring of endophytic Brenneria salicis in willow and its relation to watermark disease

M. Maes, S. Baeyen, H. De Croo, K. De Smet, M. Steenackers
1 Agricultural Research Centre, Department of Crop Protection, 9820 Merelbeke, Belgium
2 Institute of Forestry and Game Management, 9500 Geraardsbergen, Belgium E-mail: m.maes@clo.fgov.be

B. salicis was monitored in willow stands, sampling trees with and without watermark disease. The study focused on Salix alba and S. fragilis. B. salicis - presence was shown not to be restricted to diseased trees. With a B. salicis-specific PCR, B. salicis was frequently detected in the wood of healthy willows. This phenomenon was further studied with PCR-DGGE of endophytic bacterial populations in healthy and diseased willow. B. salicis concentrations were fluctuating during the year, synchronized with tree activity. Water stagnation in the wood as in winter was associated with a high B. salicis concentration, while the sap stream in active trees drained B. salicis from the wood vessels towards the leaves. We concluded that disease risk can not be assessed by testing the presence of B. salicis.

Keywords: Brenneria salicis; watermark disease; willow; endophyte; PCR-DGGE

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Maes M, Baeyen S, De Croo H, De Smet K, Steenackers M. Monitoring of endophytic Brenneria salicis in willow and its relation to watermark disease. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):528-530. doi: 10.17221/10545-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. DE KAM M. (1984): Het vaststellen van de gevoeligheid van wilgen voor de watermerkziekte: problemen en perspectieven. Nederl. Bosb. Tijdschr., 56: 22-27.
    2. D E K AM M. (1988): Watermerkziekte en de toekomst van de wilg in Nederland. Nederl. Bosb. Tijdschr., 60: 320-327.
    3. DOWSON W.J. (1937): Bacterium salicis Day, the cause of the watermark disease of the cricket bat willow. Ann. Appl. Biol., 24: 528-544. Go to original source...
    4. HAUBEN L., STEENACKERS M., SWINGS J. (1998): PCRbased detection of the causal agent of watermark disease in willows (Salix spp.). Appl. Environ. Microbiol., 64: 3966-3971. Go to original source... Go to PubMed...
    5. MUYZER G., BRINKHOFF T., NÜBEL U., SANTEGOEDS C., SCHÄFER H., WAWER C. (1998): Denaturing gradient gel electrophoresis (DGGE) in microbial ecology. Molecular Microbial Ecology Manual, 3.4.4: 1-27.
    6. VAN DER ZWEEP P., DE KAM M. (1982): The occurrence of Erwinia salicis, the cause of Watermark disease in the phyllosphere of Salix alba. Eur. J. Forest Pathol., 12: 257-261. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content