Plant Protect. Sci., 2002, 38(11):446-448 | DOI: 10.17221/10519-PPS
Oxidative burst and cell death induced by b-quercinin and zoospores of Phytophthora quercina in tobacco cell cultures
- 1 Department of Phytopathology, Center of Life Sciences, TU-München, 85350 Freising, Germany
- 2 Department of Forest Phytopathology, TU-München, 85350 Freising, Germany E-mail: j.koehl@lrz.tum.de
Mode of action of β-quercinin, a novel elicitin on tobacco cell suspension cultures (cvs. Bel B and Bel W3) was investigated by measuring the oxidative burst and cell death in these cell cultures. β-quercinin induced an oxidative burst comparable to that excited by zoospores from P. quercina. Adding superoxidedismutase, catalase and diphenyleneiodonium to elicited cell cultures, it could be demonstrated, that the induction of cell death in tobacco cell cultures is not correlated to the oxidative burst.
Keywords: P. quercina; elicitins; tobacco; oxidative burst; cell death
Published: December 31, 2002 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- ABLE A., GUEST D., SUTHERLAND M. (2000): Hydrogen peroxide yields during the incompatible interaction of tobacco suspension cells inoculated with Phytophthora nicotianae. Plant Physiol., 124: 899-910.
Go to original source... 
Go to PubMed... - BONNET P., BOURDON E., PONCHET M., BLEIN J.-P., RICCI P. (1996): Aquired resistance triggered by elicitins in tobacco and other plants. Eur. J. Plant Pathol., 102: 181-192. Go to original source...
- BOURQUE S., PONCHET M., BINET M.-N., RICCI P., PUGIN A., LEBRUN-GARCIA A. (1998): Comparison of binding properties and early biological effects of elicitins in tobacco cells. Plant Physiol., 118: 1317-1326. Go to original source... Go to PubMed...
- J ABS T., T SCHÖPE M., C OLLING C., H AHLBROCK K., SCHEEL D. (1997): Elicitor-stimulated ion fluxes and O2- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley. P. Natl. Acad. Sci., 94: 4800-4805. Go to original source... Go to PubMed...
- JUNG T., COOKE D., BLASCHKE H., DUNCAN J., OßWALD W. (1999): Phytophthora quercina sp. nov., causing root rot of European oaks. Mycol. Res., 103: 785-798. Go to original source...
- RICCI P., BONNET P., HUET J., SALLANTIN M., BEAVAISCANTE F., BRUNTEAU M., BILLARD V., MICHEL G., PERNOLLET J. (1989): Structure and activity of proteins from pathogenic fungi Phytophthora eliciting necrosis and acquired resistance in tobacco. Eur. J. Biochem., 183: 555-563. Go to original source... Go to PubMed...
- RUSTERUCCI C., STALLAERT V., MILAT M., PUGIN A., RICCI P., BLEIN J. (1996): Relationship between active oxygen species, lipid peroxidation, necrosis and phytoalexin production induced by elicitins in Nicotiana. Plant Physiol., 111: 885-891. Go to original source... Go to PubMed...
- SASABE M., TAKEUCHI K., KAMOUN S., ICHINOSE Y., G OVERS F., T OYODA K., S HIRAIHI T., Y AMADA T. (2000): Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture. Eur. J. Biochem., 267: 5005-5013. Go to original source... Go to PubMed...
- YANO A., SUZUKI K., SHINSHI H. (1999): A signaling pathway, independent of the oxidative burst, that leads to hypersensitive cell death in cultured tobacco cells includes a serine protease. Plant J., 18: 105-109. Go to original source...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.