Plant Protect. Sci., 2002, 38(11):285-287 | DOI: 10.17221/10468-PPS

Weed hosts of phytoplasmas in the Czech Republic

P. Válová, R. Fialová, M. Navrátil, J. Fránová, M. Šimková, J. Nebesářová
1 Department of Cell Biology and Genetics, Faculty of Science, Palacký University, 783 71 Olomouc-Holice, Czech Republic
2 Institute of Plant Molecular Biology, Academy of Sciences, 370 05 České Budějovice, Czech Republic E-mail: valova@prfholnt.upol.cz

The research was focused on wild plants that represent possible sources of phytoplasma infection. Thirteen species of wild herbaceous plants with symptoms of shoot proliferation, phyllody, virescence, sterility or yellowing have been collected from different locations of the Czech Republic. To detect and identify phytoplasmas, plants were subjected to electron microscopy, fluorescence microscopy, PCR, and RFLP analysis. There were phytoplasma positive species: Matricaria chamomilla, Plantago lanceolata, Plantago media, Silene latifolia ssp. alba, Stellaria media, Taraxacum officinale, Thlaspi arvense, Tragopogon pratensis, Trifolium pratense, Trifolium repens, Trifolium hybridum, Tripleurospermum maritimum, and Verbascum densiflorum. All the phytoplasmas detected were identified as belonging to the aster yellows group (subgroup 16SrI-B and 16SrI-C). With regard to a relatively high incidence of positively tested plants from several locations, Trifolium species are considered to be one of the most important natural reservoirs of aster yellows phytoplasma within the Czech Republic.

Keywords: Matricaria chamomilla; Plantago lanceolata; Plantago media; Silene latifolia ssp. alba; Stellaria media; Taraxacum officinale; Thlaspi arvense; Tragopogon pratensis; Trifolium pratense; Trifolium repens; Trifolium hybridum; Tripleurospermum maritimum; Verbascum densiorum; aster yellows phytoplasma; PCR; RFLP; TEM; DAPI staining

Published: December 31, 2002  Show citation

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Válová P, Fialová R, Navrátil M, Fránová J, Šimková M, Nebesářová J. Weed hosts of phytoplasmas in the Czech Republic. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):285-287. doi: 10.17221/10468-PPS.
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