Plant Protect. Sci., 2002, 38(11):249-251 | DOI: 10.17221/10458-PPS

Detection of Peach latent mosaic viroid by PCR

P. Ryšánek, M. Zouhar, M. Hassan
Department of Plant Protection, Czech University of Agriculture,
1 65 21 Prague-Suchdol, Czech Republic

Peach latent mosaic viroid (PLMVd) is widespread in peach all over the world. It has never been reported from the Czech Republic. That is why we adapted specific and sensitive method for its detection, PCR, to be able to prove its possible occurrence and for certification purposes. Primers PLMVdR, PLMVdF1 and PLMVdF2 were designed on the basis of published RNA sequences. Products of amplification are 208 and 114 bp long for PLMVdF1 and PLMVdF2, respectively. Four PLMVd isolates from Dr Di Serio (CNR Bari) were used as standards. Potato spindle tuber viroid and Hop latent viroid infected plant material and also healthy material were used to check detection specifity. Both RNA extraction from plant material and PCR were optimalized so that this method of PLMVd detection can also be used for certification purposes.

Keywords: PLMVd; PCR; peach; certification

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ryšánek P, Zouhar M, Hassan M. Detection of Peach latent mosaic viroid by PCR. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):249-251. doi: 10.17221/10458-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. DESVIGNES J.C. (1986): Peach latent mosaic and its relation to peach mosaic and peach yellow mosaic diseases. Acta Hortic., 183: 89-91. Go to original source...
    2. FAGGIOLI F., LORETI S., BARBA M. (1997): Occurrence of peach latent mosaic viroid (PLMVd) on plum in Italy. Plant Dis., 81: 423. Go to original source... Go to PubMed...
    3. FLORES R., LLACER G. (1988): Isolation of a viroid-like RNA associated with peach latent mosaic disease. Acta Hortic., 235: 325-332. Go to original source...
    4. HADIDI A., GIUNCHEDI L., SHAMLOUL A.M., POGGI-POLLINI C., AMER M.A. (1997): Occurrence of peach latent mosaic viroid in stone fruits and its transmission with contaminated blades. Plant Dis., 81: 154-158. Go to original source... Go to PubMed...
    5. ROBERTSON N.L., FRENCH R., GRAY S. (1991): Use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses. J. Gen. Virol., 72: 1473-1477. Go to original source... Go to PubMed...
    6. ROBINSON D.L. (1992): Detection of tobacco rattle virus by reverse transcription and polymerase chain reaction. J. Virol. Meth., 40: 57-66. Go to original source... Go to PubMed...
    7. RYŠÁNEK P. (1999): Rhizománie cukrové řepy. [Habilitační práce.] ČZU, Praha.
    8. SHAMLOUL A.M., HADIDI A. (1999): Sensitive detection of potato spindle tuber viroid and temperate fruit tree viroids by reverse transcription-polymerase chain reaction-probe capture hybridization. J. Virol. Meth., 80: 145-155. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content