Hort. Sci. (Prague), 2017, 44(3):120-125 | DOI: 10.17221/276/2015-HORTSCI

Determination of antioxidant activities of some apple cultivarsOriginal Paper

Lütfi Pirlak*, Gülüstan Ünüvar, Nilda Ersoy
Selçuk University, Faculty of Agriculture, Department of Horticulture, Selçuklu, Konya, Turkey

This study was conducted in order to determine the antioxidant activities of certain apple varieties. 'Galaxy Gala', 'Scarlet Spur', 'Fuji', 'Pink Lady' and 'Granny Smith' were characterized in this study. The antioxidant activities among the varieties were determined via three different procedures. Antiradical activity figures of the apple varieties were between 0.592 ('Galaxy Gala') and 0.802 ('Scarlet Spur'). Inhibition levels of chelating activity with Fe+2 varied between 40.20-55.50%; 'Scarlet Spur' and 'Pink Lady' extracts had the highest inhibition with 55.50% and 'Granny Smith' extract had the lowest with the level. Among the extracts showing H2O2 clearance effect, Granny Smith (62.54%) and Scarlet Spur (44.67%) extracts had the highest inhibition levels. In this study conducted on apple varieties, total phenolic matter levels were determined in 'Scarlet Spur', 'Pink Lady', 'Fuji', 'Galaxy Gala' and 'Granny Smith'.

Keywords: antiradical activity; phenolics; DPPH

Published: September 30, 2017  Show citation

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Pirlak L, Ünüvar G, Ersoy N. Determination of antioxidant activities of some apple cultivars. Hort. Sci. (Prague). 2017;44(3):120-125. doi: 10.17221/276/2015-HORTSCI.
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    References

    1. Altun M.L., Çitoğlu G.S., Yilmaz B.S., Çoban T. (2007): Antioxidant properties of Viburnum opulus and Viburnum lantana growing in Turkey. International Journal of Food Science and Nutrition, 5: 1-6.
    2. Anonymous (2013): The R Project for Statistical Computing. Available at http://www.r-project.org/
    3. Block G., Patterson B., Subar A. (1992): Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence. Nutrition and Cancer, 18:1-29. Go to original source... Go to PubMed...
    4. Boyer J., Liu R.H. (2004): Apple phytochemicals and their health benefits. Nutrition Journal, 3: 5. Go to original source... Go to PubMed...
    5. Brand-Williams W., Cuvelier M.E., Berset C. (1995): Use of freeradical method to evaluate antioxidant activity. Lebensmittel Wissenschaft und Technologie, 28: 25-30. Go to original source...
    6. Couladis M., Tzakou O., Verykokidou E., Harvala C. (2003): Screening of some Greek aromatic plants for antioxidant activity. Phytotherapy Research, 17: 194-195. Go to original source... Go to PubMed...
    7. Çoban T., Saltan Çitoğlu G., Sever B., Scan M. (2003): Antioxidant activities of plants used in traditional medicine in Turkey. Pharmaceutical Biology, 41: 608-613. Go to original source...
    8. Da Silva Porto P., Laranjinha J., De Freitas V. (2003): Antioxidant protection of low density lipoprotein by procyanidins: structure/ activity relationships. Biochemical Pharmacology, 66: 947-954. Go to original source... Go to PubMed...
    9. Drogoudi D.P., Michailidis Z., Panelidis G. (2008): Peel and flesh antioxidant content and harvest quality characteristics of seven apple cultivars. Scientia Horticulturae, 115: 149-153. Go to original source...
    10. Escarpa A., Gonzalez M.C. (1998): High-performance liquid chromatography with diode-array detection for the determination of phenolic compounds in peel and pulp from different apple varieties. Journal of Chromatograhy A, 823: 331-337. Go to original source... Go to PubMed...
    11. Eskin N.A.M., Henderson H.M., Townsend R.J. (1976): Biochemie der Lebensmittel. Hüthig Verlag.
    12. Griffits H.R., Lunec J., Arouma O.I., Halliwell B. (1998): Molecular Biology of Free Radicals in Human Diseases. London, Oica International.
    13. Halliwell B. (2001): Food-derived antioxidants: How to evaluate their importance in food and in vivo. Handbook of Antioxidants. Los Angeles, New York, CRC Press. Go to original source...
    14. Imeh U., Khokhar S. (2002): Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations. Journal of Agricultural and Food Chemistry, 50: 6301-6306. Go to original source... Go to PubMed...
    15. Karaman Ş. (2008): Türkiye'de yetiştirilen bazi elma çeşitlerinin toplam antioksidan kapasitelerinin ve antioksidan özellik gösteren başlica bileşenlerinin karşilaştirilmasi. [MSc Thesis.] İstanbul Üniversitesi Fen Bilimleri Enstitüsü Kimya Anabilim Dali, İstanbul, 116-127.
    16. Korban S.S., Skirvin R.M. (1984): Nomenclature of the Cultivated Apple. HortScience, 19: 177-180. Go to original source...
    17. Lee K.W., Kim Y.J., Kim D., Lee H.J., Lee C.Y. (2003): Major phenolics in apple and their contribution to the total antioxidant capacity. Journal of Agricultural and Food Chemistry, 51: 6516-6520. Go to original source... Go to PubMed...
    18. Leontowicz H., Gorinstein S., Lojek A., Leontowicz M., Ciz M., Soliva-Fortuny R., Park Y., Jung S., Trakhtenberg S., Martin-Belloso O. (2002): Comparative content of some bioactive compounds in apples, peaches, and pears and their influence on lipids and antioxidant capacity of rats. The Journal of Nutritional Biochemistry, 13: 603-610. Go to original source... Go to PubMed...
    19. Liu R.H. (2003): Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. American Journal of Clinical Nutrition, 78(3 Suppl): 517S-520S. Go to original source... Go to PubMed...
    20. Miller N.J., Rice-Evans C.A. (1996): The relative contributions of ascorbic acid and phenolic antioxidants to the antioxidant activity of orange and apple frui juices and blackcurrant drink. Food Chemistry, 60: 331-337. Go to original source...
    21. Molyneux P. (2004): The use of the stable free radical diphenylpicryl-hydrazl (DPPH) for estimating antioxidant activity. Songklanakarn Journal of Science and Technology, 26: 211-219.
    22. Oh H., Hoff J.E. (1987): pH dependence of complex formation between condensed tannins and proteins. Journal of Food Science, 52: 1267-1269. Go to original source...
    23. Rice-Ivens C.A., Miller N.J., Paganga G. (1997): Antioxidant properties of phenolic compounds. Trends in Plant Science, 2: 152-159. Go to original source...
    24. Rival S.G., Boeriu C.G., Wichers H.J. (2001): Caseins and casein hydrolysates antioxidative properties and relevance to lipoxygenase inhibition. Journal of Agricultural and Food Chemistry, 49: 295-302. Go to original source... Go to PubMed...
    25. Ruch R.J., Cheng S.J., Klauning J.E. (1989): Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10: 1003-1008. Go to original source... Go to PubMed...
    26. Sandra M.H. (2004): Potential impact of strawberries on human health. A rewiev of the science. Critical Reviews in Food Science and Nutrition, 44: 1-17. Go to original source... Go to PubMed...
    27. Singleton V.L., Rossi J.R. (1965): Colorimetry of total phenolics with phosphomolibdic-phosphothungstic acid. American Journal of Enology Viticulture, 16: 144-158. Go to original source...
    28. Stadtman E.R. (2002): Importance of individuality in oxidative stress and aging. Free Radical Biolog and Medicine, 33: 597-604. Go to original source... Go to PubMed...
    29. Sun J.C., Yi-Fang W., Xianzhong L., Rui H. (2002). Antioxidant and antiproliferative activities of common fruits. Journal of Agriculture and Food Chemistry, 50: 7449-7454. Go to original source... Go to PubMed...
    30. Tepe B., Sökmen M., Akpulat H.A., Sökmen A. (2006): Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chemistry, 95: 200-204. Go to original source...
    31. Vinson J.A., Zubik L., Bose P., Samman N., Proch J. (2005): Dried fruits: Excellent in vitro and in vivo antioxidants. Journal American College of Nutrition, 24: 44-50. Go to original source... Go to PubMed...
    32. Yen G.C., Wu J. (1999): Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae. Food Chemistry, 65: 375-379. Go to original source...

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