Czech J. Anim. Sci., 2015, 60(3):97-104 | DOI: 10.17221/8074-CJAS

Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattleOriginal Paper

M. Pasandideh1, M.R. Mohammadabadi2, A.K. Esmailizadeh2, A. Tarang3
1 Young Researchers and Elite Club, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
2 Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
3 Department of Genomics, Branch of North Region of Iran (Rasht), Agricultural Biotechnology Research Institute of Iran, Rasht, Iran

Several studies have reported quantitative trait loci on chromosome 6 affecting milk production and composition traits in dairy cattle. Osteopontin (OPN) and peroxisome proliferator activated receptor-γ coactivator-1α (PPARGC1A) genes have been located on this chromosome and identified as positional candidates for milk traits. We investigated the associations of single nucleotide polymorphism (SNP) T>C at position 1892 and SNP A>C at position 3359 in PPARGC1A gene as well as SNP C>T at position 8514 in OPN gene with milk production and composition. Hence, 398 Iranian Holstein cows were genotyped through polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The Least Squares methods were used to examine the effects of genotypes on milk traits. The frequencies of the C allele at position 1892, A allele at position 3359, and T allele at position 8514 were 0.56, 0.64, and 0.53, respectively. In this study, c.1892T>C genotypes indicated significant associations with milk fat content adjusted for two milkings per day (FATP2X; %), estimated breeding value for milk (EBVM; kg), milk protein yield adjusted for mature body weight (PROME; kg), milk protein yield adjusted for 305 days (PRO305; kg) (P < 0.05), and estimated breeding value for milk fat content (EBVFP; %) (P < 0.01). There were significant associations between c.3359A>C genotypes and FATP2X, EBVFP (P < 0.01). Moreover, significant associations were shown between c.8514C>T genotypes in OPN gene and FATP2X (P < 0.05), and PROPER305 (P < 0.01). Thus, these SNPs would provide an excellent opportunity for marker assisted selection programs in dairy cattle.

Keywords: cattle; OPN; PCR-RFLP; PPARGC1A; SNPs

Published: March 31, 2015  Show citation

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Pasandideh M, Mohammadabadi MR, Esmailizadeh AK, Tarang A. Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle. Czech J. Anim. Sci. 2015;60(3):97-104. doi: 10.17221/8074-CJAS.
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